Present book is written with a view to help undergraduate and postgraduate students learn the fundamental principles and applications of different types of chromatography. The author aim to facilitate the understanding of chromatographic techniques, and in detail about HPLC Techniques, Instrumentation with steps involved in New method development process through description of techniques in a simple manner. Salient features · The book explains Basic Principles of Chromatographic process, Theory & Practical techniques, and description of modern HPLC & UPLC chromatographic instrumentation and applications of these techniques. · This book will serve students in developing their research interests in the field of HPLC chromatography.